BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA

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dc.date.accessioned	2019-10-13T11:14:38Z
dc.date.available	2019-10-13T11:14:38Z
dc.date.issued	2005-04-01
dc.identifier.citation	S. M T. Hassan, M. Maache, R. Díaz de la Guardia, O. M. Córdova, J. R. Gil García, M. Galiana, D. Acuña Castroviejo, M. Martins, and Antonio Osuna "BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA," Journal of Parasitology 91(2), 284-292, (1 April 2005). https://doi.org/10.1645/GE-3352	en_US
dc.identifier.uri	http://dspace.hebron.edu:80/xmlui/handle/123456789/244
dc.description.abstract	We describe here a fatty acid–binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 × 10−8 M when the labeled oleic acid was displaced by a 10-fold greater concentration of unlabeled oleic acid. Testosterone, sodium desoxycholate, taurocholate, metronidazol, and α-tocopherol, together with butyric, arachidonic, palmitic, retinoic, and glycocholic acids, were also bound to the protein. Assays with polyclonal antibodies revealed that the protein is located in the ventral disk and also appears in the dorsal membrane, the cytoplasm, and in the vicinity of the lipid vacuoles.	en_US
dc.language.iso	en_US	en_US
dc.publisher	American Society of Parasitologists	en_US
dc.title	BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA	en_US
dc.type	Article	en_US