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dc.contributor.authorHuber, René-
dc.contributor.authorAbedalkhader/Attili, Rozan-
dc.contributor.authorKüper, Daniela-
dc.contributor.authorHauke, Lara-
dc.contributor.authorLüns, Bernadette-
dc.contributor.authorBrand, Korbinian-
dc.contributor.authorWeissenborn, Karin-
dc.contributor.authorLichtinghagen, Ralf-
dc.date.accessioned2019-07-15T09:32:38Z-
dc.date.available2019-07-15T09:32:38Z-
dc.date.issued2019-03-30-
dc.identifier.urihttp://dspace.hebron.edu:80/xmlui/handle/123456789/34-
dc.description.abstractBlood sampling with different anticoagulants alters matrix metalloproteinase (MMP-) 9 expression, thus influencing its concentration and diagnostic validity. Here, we aimed to evaluate the effects of different anticoagulants on MMP-9 regulation. MMP-9 expression was assessed in response to ethylene diamine tetra acetic acid, citrate, and high-/low-molecular-weight heparin (HMWH, LMWH) in co-culture experiments using THP-1, Jurkat, and HT cells (representing monocytes, T, and B cells). Triple and double cell line co-culture experiments revealed that HMWH treatment of THP-1 and Jurkat led to a significant MMP-9 induction, whereas other anticoagulants and cell type combinations had no effect. Supernatant of HMWH-treated Jurkat cells also induced MMP-9 in THP-1 suggesting monocytes as MMP-9 producers. HMWH-induced cytokine/chemokine secretion was assessed in co-culture supernatant, and the influence of cytokines/chemokines on MMP-9 production was analyzed. These experiments revealed that Jurkat-derived IL-16 and soluble intercellular adhesion molecule (sICAM-) 1 are able to induce MMP-9 and IL-8 production by THP-1. As a consequence, the increased MMP-9 expression found in HMWH blood samples may be influenced by HMWH-dependent secretion of IL-16 and sICAM-1 by T cells resulting in an increased production of MMP-9 and IL-8 by monocytes. IL-8, in turn, may support MMP-9 and its own expression in a positive autocrine feedback loop.en_US
dc.language.isoen_USen_US
dc.publisherInternational Journal of Molecular Scienceen_US
dc.subjectblood sampling; anticoagulants; MMP-9; high-molecular-weight heparin; IL-16; sICAM-1; IL-8; monocytes; T cellsen_US
dc.titleCellular and Molecular Effects of High-Molecular-Weight Heparin on Matrix Metalloproteinase 9 Expressionen_US
dc.typeArticleen_US
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