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http://dspace.hebron.edu:8080/xmlui/handle/123456789/248| Title: | BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA |
| Authors: | Hassan SM T |
| Issue Date: | 1-Apr-2005 |
| Publisher: | American Society of Parasitologists |
| Citation: | S. M T. Hassan, M. Maache, R. Díaz de la Guardia, O. M. Córdova, J. R. Gil García, M. Galiana, D. Acuña Castroviejo, M. Martins, and Antonio Osuna "BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA," Journal of Parasitology 91(2), 284-292, (1 April 2005). https://doi.org/10.1645/GE-3352 |
| Abstract: | We describe here a fatty acid–binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 × 10−8 M when the labeled oleic acid was displaced by a 10-fold greater concentration of unlabeled oleic acid. Testosterone, sodium desoxycholate, taurocholate, metronidazol, and α-tocopherol, together with butyric, arachidonic, palmitic, retinoic, and glycocholic acids, were also bound to the protein. Assays with polyclonal antibodies revealed that the protein is located in the ventral disk and also appears in the dorsal membrane, the cytoplasm, and in the vicinity of the lipid vacuoles. |
| URI: | http://dspace.hebron.edu:80/xmlui/handle/123456789/248 |
| Appears in Collections: | Proposals |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Immunolocalization.pdf | 747.21 kB | Adobe PDF | View/Open |
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